ATP Synthase alpha (AA 113-220) anticorps

N° du produit ABIN968856

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans IF et WB. Adapté pour Poulet, Chien, Humain, Souris et Rat. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour ATP Synthase alpha (AA 113-220) anticorps (ABIN968856)

Antigène: ATP Synthase alpha

Reactivité: Poulet, Chien, Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Clone: 51-ATP Synthase alpha

Détail du produit

Épitope: AA 113-220

Réactivité croisée: Chien, Rat (Rattus), Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human ATP Synthase alpha aa. 113-220

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Référence

von Ballmoos, Appoldt, Brunner, Granier, Vasella, Dimroth: "Membrane topography of the coupling ion binding site in Na+-translocating F1F0 ATP synthase." dans: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3504-10, (2002) (PubMed).

Xu, Zanotti, Gaballo, Raho, Papa: "F1 and F0 connections in the bovine mitochondrial ATP synthase: the role of the of alpha subunit N-terminus, oligomycin-sensitivity conferring protein (OCSP) and subunit d." dans: European journal of biochemistry / FEBS, Vol. 267, Issue 14, pp. 4445-55, (2000) (PubMed).

Lee, Garboczi, Thomas, Pedersen: "Mitochondrial ATP synthase. cDNA cloning, amino acid sequence, overexpression, and properties of the rat liver alpha subunit." dans: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4664-9, (1990) (PubMed).

Détail du antigène

Antigène: ATP Synthase alpha

Sujet: ATP synthase is a large enzyme complex that uses an electrochemical H+ or Na+ gradient to synthesize ATP from ADP and Pi, providing the organism with the ATP needed for energy. The complex consists of two major units, F0 and F1. F0 is embedded in the inner membrane of the mitochondria and, due to its hydrophobic nature, translocates protons across this membrane. F1 is the catalytic portion in the matrix region of the mitochondria and is comprised of alpha, beta, gamma, delta, and epsilon subunits at a 3:3:1:1:1 ratio. The alpha subunit is a ubiquitous protein that is highly conserved among species. It has an adenine specific binding site that binds both ATP and ADP. There are two glycine rich regions in the sequence, the A domain and B domain, that are thought to be part of the nucleotide binding domain. It has been demonstrated that the N-terminus of the alpha subunit is necessary for the correct functional and structural connection of F0 to F1. Thus, the alpha subunit is an essential component of the ATP synthase complex and plays a role in properly orienting the F0 and F1 units.

Poids moléculaire: 55 kDa